裙带菜多糖硫酸酯诱导人肝癌HepG-2细胞凋亡机制研究

杨波, 王福玲, 季宇彬, 耿豪滨, 伊亚彤, 刘丽, 江蔚新, 赵威

中国药学杂志 ›› 2017, Vol. 52 ›› Issue (21) : 1909-1913.

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中国药学杂志 ›› 2017, Vol. 52 ›› Issue (21) : 1909-1913. DOI: 10.11669/cpj.2017.21.008
论著

裙带菜多糖硫酸酯诱导人肝癌HepG-2细胞凋亡机制研究

  • 杨波a, b, 王福玲a, b, 季宇彬b, 耿豪滨a, 伊亚彤a, 刘丽a, 江蔚新a, 赵威a
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Apoptotic Mechanisms of Human Liver Cancer HepG-2 Cells Induced by Undaria pinnatifida Sulfated Polysaccharides

  • YANG Boa, b, WANG Fu-linga, b, JI Yu-binb, GENG Hao-bina, YI Ya-tonga, LIU Lia, JIANG Wei-xina, ZHAO Weia
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摘要

目的 探讨裙带菜多糖硫酸酯S-UPPSⅠB体外诱导人肝癌HepG-2细胞凋亡的作用及机制。方法 采用四甲基偶氮唑蓝(MTT)法检测S-UPPSⅠB对HepG-2细胞增殖作用的影响;激光共聚焦显微镜测定HepG-2细胞内[Ca2+]i水平;流式细胞仪测定细胞凋亡率及Bcl-2、Bax、Cyt-c、p53的表达;Caspase-3, -9试剂盒检测蛋白表达。结果 S-UPPSⅠB 抑制HepG-2细胞的IC50值为50.09 μgmL-1, 随着给药剂量的增加, 凋亡率亦增加, 并且对Ca2+及其通路的相关蛋白有明显的调节作用, 其中[Ca2+]i的水平及Cyt-C、Caspase-3, -9、p53的表达均有显著增加(P<0.05), Bcl-2/Bax比值降低。结论 裙带菜多糖硫酸酯S-UPPSⅠB可显著抑制人肝癌HepG-2细胞增殖, 并可通过启动线粒体途径诱导HepG-2细胞发生凋亡。

Abstract

OBJECTIVE To investigate the mechanism of human liver cancer HepG-2 cells apoptosis induced by Undaria pinnatifida sulfated polysaccharides S-UPPSⅠB. METHODS The anti-proliferation effects of S-UPPSⅠB on HepG-2 cells was by MTT assay. [Ca2+]i in HepG-2 cells was detected by laser cofocal scaning microscopy (LCSM). The apoptosis rate and protein expression level of Bcl-2, Bax, Cyt-C and p53 were detected by flow cytometry (FCM). Caspase Assay Kit was used to detected the activities of Caspase-3 and -9. RESULTS S-UPPSⅠB could inhibit the proliferation of HepG-2 cells and the IC50 was 50.09 μgmL-1. With the increase of drug delivery dosage, apoptosis rate also increased, and the significant regulating effects of S-UPPSⅠB on Ca2+ and its associated channel proteins were observed. The [Ca2+]i level, the protein expression level of Cyt-c, p53 and the activities of Caspase-3 and -9 were all increased remarkably (P<0.05). The ratio of Bcl-2 to Bax was reduced. CONCLUSION Undaria pinnatifida sulfated polysaccharides S-UPPSⅠB can effectively inhibit the proliferation of human liver cancer HepG-2 cells by inducing apoptosis of HepG-2 cells through mitochondrial pathway.

关键词

裙带菜 / 多糖硫酸酯 / 人肝癌HepG-2细胞 / 凋亡

Key words

Undaria pinnitafida / sulfated polysaccharide / HepG-2 / apoptosis

引用本文

导出引用
杨波, 王福玲, 季宇彬, 耿豪滨, 伊亚彤, 刘丽, 江蔚新, 赵威. 裙带菜多糖硫酸酯诱导人肝癌HepG-2细胞凋亡机制研究[J]. 中国药学杂志, 2017, 52(21): 1909-1913 https://doi.org/10.11669/cpj.2017.21.008
YANG Bo, WANG Fu-ling, JI Yu-bin, GENG Hao-bin, YI Ya-tong, LIU Li, JIANG Wei-xin, ZHAO Wei. Apoptotic Mechanisms of Human Liver Cancer HepG-2 Cells Induced by Undaria pinnatifida Sulfated Polysaccharides[J]. Chinese Pharmaceutical Journal, 2017, 52(21): 1909-1913 https://doi.org/10.11669/cpj.2017.21.008
中图分类号: R965   

参考文献

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基金

黑龙江省教育厅面上项目资助(12521134);国家级大学生创新训练计划项目资助(201610240025)
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